BAD antibody (pSer99)

Catalog No. ABIN3030130

This Rabbit Polyclonal antibody specifically detects BAD in ELISA, IHC, DB and IF. It exhibits reactivity toward Human.

Quick Overview for BAD antibody (pSer99) (ABIN3030130)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Dot Blot (DB), Immunofluorescence (IF)

Product Details anti-BAD Antibody

Binding Specificity: pSer99

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Purification: Antigen affinity purified

Immunogen: This phospho-Bad antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS99 of human Bad.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-Bad antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:200,Dot blot: 1:500,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-Bad antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: Bad

Background: Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events